UNG Antikörper (AA 96-313)

Produktnummer ABIN7602695

Dieser Anti-UNG Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von UNG in WB, IHC, ELISA, IF, ICC und FACS. Geeignet für Human, Maus, Ratte und Affe.

Kurzübersicht für UNG Antikörper (AA 96-313) (ABIN7602695)

Target: UNG (Uracil-DNA Glycosylase (UNG))

Reaktivität: Human, Maus, Ratte, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser UNG Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-UNG Antikörper

Bindungsspezifität: AA 96-313

Verwendungszweck: Anti-UNG Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-UNG Antibody Picoband® (ABIN7602695). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human UNG recombinant protein (Position: E96-L313).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat, Monkey
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Lindahl T, Ljungquist S, Siegert W, Nyberg B, Sperens B (May 1977). "DNA N-glycosidases: properties of uracil-DNA glycosidase from Escherichia coli". The Journal of Biological Chemistry. 252 (10): 3286-94. 2. Longo MC, Berninger MS, Hartley JL (Sep 1990). "Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reactions". Gene. 93 (1): 125-8. 3. Pearl LH (Aug 2000). "Structure and function in the uracil-DNA glycosylase superfamily". Mutation Research. 460 (3-4): 165-81.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu UNG

Target: UNG (Uracil-DNA Glycosylase (UNG))

Andere Bezeichnung: UNG

Hintergrund:

Synonyms: Phosphatidylethanolamine-binding protein 1, PEBP-1, HCNPpp, Neuropolypeptide h3, Prostatic-binding protein, Raf kinase inhibitor protein, RKIP, Hippocampal cholinergic neurostimulating peptide, HCNP, PEBP1, PBP, PEBP

Tissue Specificity: Expressed in kidney, skeletal muscles, liver, lung, breast, intestine, placenta and skin mainly in epithelial cells (at protein level).

Background: Uracil-DNA glycosylase, also known as UNG or UDG, is a human gene though orthologs exist ubiquitously among prokaryotes and eukaryotes and even in some DNA viruses. The first uracil DNA-glycosylase was isolated from Escherichia coli. This gene encodes one of several uracil-DNA glycosylases. One important function of uracil-DNA glycosylases is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. Uracil bases occur from cytosine deamination or misincorporation of dUMP residues. Alternative promoter usage and splicing of this gene leads to two different isoforms: the mitochondrial UNG1 and the nuclear UNG2. The UNG2 term was used as a previous symbol for the CCNO gene, which has been confused with this gene, in the literature and some databases.

Molekulargewicht: 35 kDa

Gen-ID: 7374

UniProt: P13051

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response